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PBSD MSTBU Agenda 10/30/2023PELICAN BAY SERVICES DIVISION Municipal Service Taxing and Benefit Unit NOTICE OF PUBLIC MEETING OCTOBER 30, 2023 THE CLAM BAY COMMITTEE OF THE PELICAN BAY SERVICES DIVISION WILL MEET AT 1:30 PM ON MONDAY, OCTOBER 30 AT THE TRUIST BANK BUILDING, 801 LAUREL OAK DRIVE, SUITE 102, NAPLES, FLORIDA 34108. AGENDA 1. Pledge of Allegiance 2. Roll call 3. Agenda approval 4. Approval of 5/2/2023 meeting minutes 5. Audience comments 6. "Approval of draft plans for proposed 2024 dredging of Clam Pass 7, 2022 Clam Bay Water Quality Report 8. Adjournment "indicates possible action items ANY PERSON WISHING TO SPEAK ON AN AGENDA ITEM WILL RECEIVE UP TO THREE (3) MINUTES PER ITEM TO ADDRESS THE BOARD. THE BOARD WILL SOLICIT PUBLIC COMMENTS ON SUBJECTS NOT ON THIS AGENDA AND ANY PERSON WISHING TO SPEAK WILL RECEIVE UP TO THREE (3) MINUTES. THE BOARD ENCOURAGES YOU TO SUBMIT YOUR COMMENTS IN WRITING IN ADVANCE OF THE MEETING. ANY PERSON WHO DECIDES TO APPEAL A DECISION OF THIS BOARD WILL NEED A RECORD OF THE PROCEEDING PERTAINING THERETO, AND THEREFORE MAY NEED TO ENSURE THAT A VERBATIM RECORD IS MADE, WHICH INCLUDES THE TESTIMONY AND EVIDENCE UPON WHICH THE APPEAL IS TO BE BASED, IF YOU ARE A PERSON WITH A DISABILITY WHO NEEDS AN ACCOMMODATION IN ORDER TO PARTICIPATE IN THIS MEETING YOU ARE ENTITLED TO THE PROVISION OF CERTAIN ASSISTANCE. 10/24/2023 11:26 AM PELICAN BAY SERVICES DIVISION CLAM BAY COMMITTEE MEETING MAY 2, 2023 The Clam Bay Committee of the Pelican Bay Services Division met on Tuesday, May 2 at 1:00 p.m. at the PBSD Office in the Truist Building, 801 Laurel Oak Drive, Naples, Florida 34108. In attendance were: Clam Bay Committee Denise McLaughlin, Chair Pelican Bay Services Division Staff Neil Dorrill, Administrator Chad Coleman, Operations Manager Darren Duprey, Supervisor — Field I (absent) Also Present Mohamed Dabees, Humiston & Moore Michael Rodburg (absent) Rick Swider Dave Greenfield, Supervisor -Field II Karin Herrmann, Project Manager I Lisa Jacob, Project Manager II Barbara Shea, Admin. Support Spec. II Michael Fogg, PBSD Board APPROVED AGENDA (AS PRESENTED) 1. Pledge of Allegiance 2. Roll can 3. Agenda approval 4. Approval of 1/26/2023 meeting minutes 5. Audience comments 6. Collier County Coastal Storm Risk Management Study Update (Dr. Dabees) 7. Update on Clam Bay tide gauges 8. Adjournment ROLL CALL Mr. Rodburg was absent and a quorum was established. Mr. Swider motioned, Ms. McLaughlin seconded to approve the agenda as The motion carried Mr. Swider motioned, Ms. McLaughlin seconded to approve the I/26/2023 meeting minutes as presented. The motion carried unanimously. Pelican Bay Services Division Clam Bay Committee Meeting May 2, 2023 AUDIENCE COMMENTS Ms. Ashley Jenkins and Ms. Shelley McKernan introduced themselves and commented that they attended the April Big Cypress Basin Board April meeting as well as the USACE (U.S. Army Corps of Engineers) public meetings last week on the Collier County Coastal Storm Risk Management Study. They commented that they are advocates for water quality improvements in the County and are studying these issues and possible solutions. Ms. Jenkins and Ms. McKernan stated that they do not represent any organization, and that they are attending this meeting for educational purposes. Dr. Dabees commented that Clam Bay is a natural preserve area, and suggested that it is an example of how coastal communities should be developed. He noted that the PBSD continuously manages the mangroves and the bay, and that we have a water quality monitoring system in place. COLLIER COUNTY COASTAL STORM RISK MANAGEMENT STUDY UPDATE Dr. Mohamed Dabees, Coastal Engineer with Humiston &Moore, provided an update on what was learned at last week's USACE 3-day chan•ette (for stakeholders) and two public meetings on the Collier County Coastal Storm Risk and Resiliency Management Study. Highlights included the following. • The USACE provided a review of their goals for this study. As their process goes forward, they are hoping to improve communication and engagement with stakeholders and the public. There was a lot of public interest at these public meetings. • Mr. Dorrill, representing the PBSD and Mr. Hoppensteadt, representing the PBF attended the charrette. • Our point of contact for this study going forward will be the County's new Director of Community Planning and Resiliency, Chris Mason. An advisory body will be formed with members representing all areas of the County as well as the two cities. • Mr. Dorrill and Mr. Hoppensteadt plan to schedule a meeting with Mr. Mason to discuss Pelican Bay's interests and concerns relating to the study. • The Collier County CAC (Coastal Advisory Committee) was informed that going forward any CAC members will be speaking as individuals (and not as committee members) on this study. • Although Dr. Dabees represented the City of Marco Island at the charr•ette, he participated in discussions regarding Clam Pass & Clam Bay. • Dr. Dabees will provide feedback to the USACE as solutions are considered and pursued, going forward. He suggested that the installation of gates and/or walls in Clam Pass/Clam Bay would result in significant environmental impacts, and therefore, not recommended. Environmental impacts must be considered for any proposed projects. • The USACE's goal is to complete a draft report by year end and complete a final report of recommendations by 2025. • The USACE study and report are 100% federally funded. The execution of any recommended projects would be 65% federally funded and 35% County funded. Ms. McLaughlin commented that going forward, the PBSD's role will be to educate ourselves, communicate with our representative Mr. Mason, and provide communication to our community. UPDATE ON CLAM BAY TIDE GAUGES 2 Pelican Bay Services Division Clam Bay Committee Meeting May 2, 2023 Dr. Dabees reported that one tide gauge was successfully installed in March, and the other three were installed in April. He noted that the gauges are not transmitting continuous data. Dr. Dabees will reach out to Locher Environmental (the system host) to have these software glitches rectified. Dr. Dabees commented that Clam Pass currently looks good and is flushing properly. He provided a recent photo of the Pass to the committee. LAST WEEKEND'S STORM Dr. Dabees reported that last weekend's storm had sustained wind and waves for two continuous days, with 3-4 foot waves and a two -foot storm surge. Mr. Coleman reported that the surge caused water to go over the County's newly installed berm, and that all of the newly installed sand at Marker 36 has been wiped out. He commented that the County has not made a decision on what action will be taken regarding the loss of their project's sand. Dr. Dabees expressed concerns on the lack of sufficient slope adjacent to the newly constructed berm. He commented that in the next 24 hours, he will make a site visit to assess the storm damage, and then make appropriate recommendations. The meeting was adjourned at 2:17 Denise McLaughlin, Chaii• Minutes approved [] as presented OR [] as amended ON ( ] date E0 a zo <Z3� / 1 N N N m W = O] U � a U 0 3+64:-, Go U= M h«MIK 5 In mo k 3 m I op omw om Oj �< I F moo 0 E 33 zm \\ s wL -z zu -WONs` Nq gw <s N O N O W O m O Z O � p On 6 mill _ V z Um~ o moo: ROE 1, Agenda Item #7 Page 1 of 20 2022 CLAM BAY WATER QUALITY ANALYSIS Water Quality Analysis for Evaluation of Limit with Numeric Nutrient Criteria SECTIONS 4/5/9/32/32, TOWNSHIPS 48/49 SOUTH, RANGE 25 EAST COLLIER COUNTY. FLORIDA Prepared For: Pelican Bay Services Division PELICAN c/o Lisa Jacob ®BAY 801 Laurel Oak Drive, Suite 302 Naples, FL 34108 Prepared By: Earth Tech Environmental, LLC 10600 Jolea Avenue Bonita Springs, FL 34135 EARTH TECH 239.304.0030 ENVIRONMENTAL www.eteflorida.com September 18, 2023 1.0 EXECUTIVE SUMMARY .................................................... Agenda Item #7 Page 2 of 20 2022 Clam Bay Water Quality Analysis .................................................... 3 2.0 LIMITATIONS AND EXCEPTIONS............................................................................................................3 3.0 BACKGROUND.......................................................................................................................................4 4.0 SITE DESCRIPTION.................................................................................................................................5 5.0 METHODOLOGY....................................................................................................................................6 5A Total Phosphorus (TP) & Total Nitrogen (TN)....................................................................................... 6 5.2 Dissolved Oxygen Saturation(DOj........................................................................................................6 5.3 Copper (Cu)..........................................................................................................................................a 6 5.4 Chlorophyll-a.........................................................................................................................................6 6.0 RESULTS.................................................................................................................................................7 6.1 Total Phosphorus & Total Nitrogen.......................................................................................................7 6.2 Dissolved Oxygen Saturation..............................................................................................................14 6.4 Copper ............................... ...........................................................................................I.I.......I............16 6.5 Chlorophyll-a.......................................................................................................................................18 7.0 DISCUSSION & RECOMMENDATIONS.................................................................................................20 8.0 REFERENCES........................................................................................................................................21 EXHIBITS Figure 1 Site Location Map Figure 2 Water Sample Locations Map Figure 3 Total Phosphorus Concentrations Figure 4 Total Nitrogen Concentrations Figure 5 Dissolved Oxygen Saturation Values Figure 6 Copper Saturation Values Figure 7 Chlorophyll -a Values Figure 8 Relationship Between Total Phosphorus and Total Nitrogen Figure 9 Relationship Between Total Phosphorus and Dissolved Oxygen Saturation Figure 10 Relationship Between Total Phosphorus and Chlorophyll -a TABLES Table 1 Total Phosphorus Concentrations Table 2 Total Nitrogen Concentrations Table 3 Dissolved Oxygen Saturation Values Table Copper Values Table 5 Chlorophyll -a Values APPENDICES Appendix A 2021 Clam Bay Water Quality Analysis Report (Stantec) LLC www.eteflorida.com Agenda Item #7 Page 3 of 20 2022 clam Bay Water Quality Analysis 1,0 EXECUTIVE SUMMARY Earth Tech Environmental (ETE) conducted data analysis of water quality samples taken from nine (9) pre- established locations within the Clam Bay Natural Resources Protection Area (NRPA), hereafter referred to as Clam Bay, Clam Bay is located in coastal Naples, Florida in Collier County, and is comprised of approximately 560 acres of estuary, mangrove forest, and hand dug channels with connectivity to the Gulf of Mexico via Clam Pass. See Figure 1 for a location map. Water quality data for Clam Bay was collected by Collier County Pollution Control (CCPC) and reported through the Florida Department of Environmental Protection's (FDEP) Watershed Information Network (WIN) from January through December 2022. Water quality data results were analyzed individually and reported for overall compliance with site -specific Numeric Nutrient Criteria (NNC) for the following five analytes: Total Nitrogen (TN), Total Phosphorus (TP), Dissolved Oxygen Saturation (DO), Copper (Cu) and Chlorophyll -a. Water quality data obtained over the duration of January through December 2022 indicated that Clam By was out of compliance for the following analytes: Total Phosphorus, Total Nitrogen, Dissolved Oxygen Saturation and Chlorophyll -a, based on limits set by the FDEP. Copper, which was banned in the City of Naples in November 2009, was within compliance. To be in compliance, no morethan 10%of samples can exceed limits set by FDEP's NNC. Total Phosphorus levels exceeded the NNC in 43/107 (40%) of samples collected. Data and prior reports show that Total Phosphorus levels have been consistently out of compliance overthe last eight years. Total Nitrogen levels exceeded the NNC in 17/107 (16%) of samples collected. Dissolved Oxygen Saturation levels exceeded the NNC in 24/108 (22%) of samples collected. Copper was out of compliance for 5/108 (0.05%) of samples collected. Chlorophyll -a levels exceeded the NNC in 36/108 (33%) of samples collected. 2.0 LIMITATIONS AND EXCEPTIONS This report is expressly for the sole and exclusive use of the party for whom this report was originally prepared for a particular purpose. Only the party for whom this report was originally prepared and/or other specifically named parties have the right to make use of and rely upon this report. Reuse of this report Crary portion thereof for otherthan its intended purpose, or if modified, or if used by third parties, shall be at the user's sole risk. Past analysis was used for reference and not prepared or re-evaluated by ETE for the purpose of this report. Earth Tech Environmental, LLC G Figure 1. Site Location Map 3.0 BACKGROUND Agenda Item #7 Page 4 of 20 2022 Clam Bay Water Quality Analysis The 2021 Clam Bay Water Quality Analysis report was prepared by Stantec, and previous reports were prepared by other entities; data presented in this 2022 report is compared to data analyzed by others and has not been re-evaluated by ETE. The 2021 report is included in Appendix A for reference. Pelican Bay Services Division (PBSD) Municipal Services Taxing & Benefit Unit was formed in part to provide water management, beach renourishment, beautification of recreation facilities, and the maintenance of conservation preserve areas. PBSD is responsible for advising Collier County on dredging and maintaining Clam Pass to ensure the health of the mangrove forest and estuary. Water quality may be affected by environmental factors such as storm activity and tidal influence, in addition to anthropogenic factors such as dredging and construction activities. The U.S. Environmental Protection Agency (EPA) adopted nutrient concentration criteria for Clam Bay in 2011, which were also reviewed and approved by the FDEP. Clam Bay has been assigned NNCs that are termed Site -Specific Alternative Criteria (SSAC), as listed in Chapter 62-302,500 of the Florida Administrative Code (F.A.C). The criteria developed for TP and TN depend on salinity and specific conductivity, since analyte concentrations in saltwatersystems varywith dynamicfactors such as rainfall, runoff, and tidal influence. In addition to considering salinity and specific conductivity of waters when evaluating whether specific analytes meet NNC, the analysis also considers the frequency with which analyte concentrations exceed NNC values, as well as the amount of time over which exceedances have occurred for the 2022 calendar year. This information may be used to help determine management responses. Clam Bay experienced a few major disturbances in 2022 including the dredging of Clam Pass in February through April 2022 and Hurricane Ian on September 28, 2022, which brought 8-10 feet of storm surge into Clam Bay. Earth Tech Environmental, LLC www.eteflorida.com CB 7 26.2379s, -81 I -'y d n 262379 .�60�11 133„ t IN I !A$�y;ef, IS CBCB 2 P' 26.237�95?I8181�133A I I t 95. t1 1 It { IA elIot CB i w,IC le�IwpZpb j' 2G 23795 81 81433; I ki V et CB It 26.2.3795, :fit BI6433 isr3 ` %1; �pi P1.' �� ear \�el L All 14 Of v I It fPi� i ��. ♦ 1 fir. •,.s � LA 0 or FlIF � A {ADZ : ,.. , ev �CB sf�\\''KK�MOIL 26 2379 81.04164 3� �@` �: a B -. , $d ky 4 <„ t MI r 9' � Iat�1 y" ac�ad XFa t I �4 26.2379 818116433 I let. Agenda Item #7 Page 6 of 20 2022 Clam Bay Water Quality Analysis 5.0 METHODOLOGY Water quality samples were collected by Collier County Pollution Control and analyzed by Collier County Pollution Control Laboratory for 17 analytes at nine sample locations within Clam Bay. Samples were collected once monthly over the 2022 calendar year. The 17 analytes included: Ammonia, Chlorophyll -a, Copper (Cu), Nitrate -Nitrite, Nitrite, Nitrogen- Total Kjeldahl (TN), Orthophosphate (P), Pheophytin a, Phosphorus- Total (TP), Dissolved Oxygen, Dissolved Oxygen Saturation (DO), pH, Salinity, Specific Conductance, Water Temperature, Depth- Secchl Disk Depth, and Turbidity. All results were obtained from WIN, including analytes that are known indicators in declaring a waterbody impaired or out of compliance by EPA. Discussed below are the results for the 2022 calendar year, which specifically focus oIn Total Phosphorus, Total Nitrogen, Dissolved Oxygen Saturation, Copper, and Chlorophyll -a. Results have been calculated and displayed below as pass/fail. 5.1 Total Phosphorus (TP) &Total Nitrogen (TN) As outlined in 62-302.531 F.A.C., "the water quality status of Total Nitrogen and Total Phosphorus is determined on an annual basis, preferably within a calendar year, based on Specific Conductivity as a dynamic qualifier." To determine concentration, samples are collected via a grab bottle and then diluted and run through a gas chromatographerto read concentration levels. Results arethen calculated persite as the limits are dependent on many factors such as the relationship between nutrients and salinity, tide, rainfall, and temperature. These factors are used to determine if the waters of Clam Bay are within compliance or not. The frequency and duration in which values exceed NNC is considered when determining appropriate management responses. The specific limits for each sampling location are calculated according to 62-302-532 F.A.C.: "No more than 10 percent of the individual Total Phosphorus (TPJ or Total Nitrogen INJ measurements shall exceed the respective TP Upper Limit or TN Upper Limit." Equation 1: TP Upper Limit(mg/L) = et-1.o62se-o.000032eaas•conda�twlryl�ll Equation 2: TN Upper Limit(mg/L) = 2.3601-0.000026R325*Conductivity(µS) 5.2 Dissolved Oxygen Saturation (DO) The amount of oxygen that can be dissolved in water is dependent on several factors, including water temperature, salinity, and atmospheric pressure. Dissolved Oxygen Saturation is recorded in the field in real time using a specialized meter that records dissolved oxygen, temperature, salinity, and atmospheric pressure. DO shall not fall below 42%saturation in more than 10 percent of the values over the calendar year as stated in 62-302.533 F.A.C. 5.3 Copper (Cu) Copper is an essential nutrient at low concentrations but is toxic to aquatic organisms at higher concentrations. A 2006 National Oceanic and Atmospheric Administration study (NOAA) suggests that higher Cu concentrations may be acutely toxic for some fish species and are lethal for most invertebrates; following this, the Florida Department of Agriculture and Consumer Services banned such uses of copper sulfate in the City of Naples in November of 2009, The limit for Copper has been set to not exceed 3.7 ug/L in compliance with 62-302-530 F.A.C. 5.4 Chlorophyll -a Chlorophyll -a is a measure of the amount of algae and phytoplankton growing in a waterbody; too much can cause algae blooms. Such blooms block light from penetrating the surface water and reduce water Earth Tech Environmental, LLC www.eteflorida.com 6 Agenda Item #7 Page 7 of 20 2022 Clam Bay Water Quality Analysis clarity. Die -offs of these blooms cause large amounts of carbon dioxide to enter the water system and decrease available oxygen. In accordance with 62-302.531 F.A.C., levels of Chlorophyll -a are not to exceed 11 ug/L. 6.0 RESULTS Below is a discussion of results based on these set parameters and calculations. Comparisons and correlations, if any, between TP and Chlorophyll -a. TP and TN, and any effect of TP on DO, are also provided. 6.1 Total Phosphorus &Total Nitrogen Total Phosphorus levels are out of compliance for 43/107 (40%) of samples collected and Total Nitrogen levels are out of compliance for 17/107 (16%) samples. Total Phosphorous increased at the northern sites in April and May 2022, and again at all of the sites in October 2022 following Hurricane Ian, Total Nitrogen increased at all sites January— March 2022, and again at 8/9 sample locations in October 2022. Nitrogen and Phosphorus are essential nutrients In the life cycles of plants and animals. However, when in excess, may cause an explosion in growth of nuisance plants and algae blooms, reducing water clarity and blocking Iightfrom reaching below the surface to seagrass beds. The excess algae and plant matter eventually decompose, producing substantial amounts of carbon dioxide. Phosphorus and Nitrogen enter waterways from rain runoff of human and animal waste, laundry and cleaning wastewater, industrial effluents, and fertilizer runoff. Soil erosion is also a major contributor of nutrients into waterways. The following figures depict results of Total Phosphorous and Total Nitrogen at each sample location. Table 1. Total Phosphorus concentrations per sample location from March 2015 through December 2022 in Clam Bay. Green represents samples in compliance with the NNC. Red cells indicate exceedance of the NNC. Red cells with an 'X" represent values that are within 5% of NNC concentration, suggesting lack of compliance should be interpreted with caution, due to analytical precision. Gray cells represent lack of data. TABLE 1. Total Phosphorous (TP) (mg/L) -" Date Sample Location (month/year) CBl CB2 CB3 CB4 CB5 CB6 CB7 C68 CB9 Mar-15 Mar-15 Apr-15 May-15 X lun-15 Jul-15 Aug-15 Sep-15 OM-15 Nov-15 X Dec-15 Jan-16 Feb-16 Earth Tech Environmental, LLC www.eteflorida.com Agenda Item #7 Page 8 of 20 2022 Clam Bay Water Quality Analysis Mar-16 Apr-16 May-16 Jun-16 Jul-16 Aug-16 Sep-16 Oct-16 Nov-16 Dec-16 Jan-17 Feb-17 Mar-17 X Apr-17 May-17 X Jun-17 Jul-17 Aug-17 Sep-17 Oct-17 Nov-17 Dec-17 Jan-18 Feb-18 Mar-18 X X Apr-18 X May-18 X Jun-18 Jul-18 Aug-18 Sep-18 Oct-18 Nov-18 Dec-18 Jan-19 Feb-19 9 X 9999999 j Earth Tech Environmental, LLC _ www.eteflorlda.com Agenda Item #7 Page 9 of 20 2022 Clam Bay Water Quality Analysis Dec-19 Jan-20 Feb-20 Mar-20 RIM Apr-20 May-20 Jun-20 Jul-20 x Aug-20 Sep-20 OM-20 Nov-20 Dec-20 Jan-21 X Feb-21 Mar-21 X x x Apr-21 May-21 Jun-21 X x x X Jul-21 Aug-21 Sep-21 Oct-21 Nov-21 Dec-21 Jan-22 79 0.073 0.059 0.048 0.046 0.052 0.033 0.034 0.053 Feb-22 16 0.076 0.058 0.043 0.046 0.052 0.059 0.058 0.065 Mar-22 02 0.125 0,127 0-11 0-099 0.101 0.12 0.084 0.089 Apr-22 13 0.102 0.098 0.035 0.041 0.069 0-092 0.078 0.081 May-22 64 0 M 0.098 0.096 0.054 0.044 0.064 0.057 0.062 Jun-22 .1 0.09 0.07 0.061 0.047 0.046 0.054 0.043 0.057 July-22 086 0.059 0.054 0.067 0.038 0.044 0.051 0.058 0.11 Aug-22 .08 0,06 0.05 0.044 0.041 0.036 0.06 0.054 0.045 Sep-22 076 0.039 0.037 0.037 0.029 0.041 0.043 0.051 0.047 Oct-22 66 0.108 0.094 0-067 0.05 0.056 0.049 0.061 0.071 Nov-22 -.61 0.181 n/a 0,096 0.082 0.063 0.071 0.078 0.057 Dec-22 55 0.141 0.101 0.115 0-085 0.096 (H 0.D81 0.131 Earth Tech Environmental, LLC _ www.e[eflorida.com Agenda Item#7 Page 10 of 20 2022 Clam Bay Water Quality Analysis Figure 3. Total Phosphorous concentrations per sample location over the period of January 2022 through December 2022 in Clam Bay, 0.3 0.25 m 0.2 0 a 0.15 0 a 0.1 F�- 0.05 C Total Phosphorus(TP) 1 2 3 4 S 6 7 8 9 10 11 12 Month t CB1 C82 C83 C84 t- CB5 F C86 t C87 t CB8 —�—CB9 Earth Tech Environmental, LLC 10 www.eteflorida.com Agenda Item #7 Page 11 of 20 2022 Clam Bay Water Quality Analysis Table 2. Total Nitrogen concentrations per sample location from March 2015 through December 2022 in Clam Bay. Green represents samples in compliance with the NNC. Red indicates exceedance of the NNC. Red cells with an 'X" represent values that are within 5% of NNC concentration, suggesting lack of compliance should be interpreted with caution, due to analytical precision. Gray cells represent lack of data. TABLE 2. Total Nitrogen (TN) (mg/L) Date Sample Location (month/year) CBl CB2 CB3 CB4 CBS CB6 CB7 CB8 CB9 Mar-15 Mar-15 Apr-15 May-15 ` Jun-15 Jul-15 Aug-15 Sep-15 ;A -- Oct-15 Nov-15 Dec-15 Jan-16 Feb-16 Mar-16 Apr-16 May-16 Jun-16 Jul-16 Aug-16 Sep-16 Oct-16 Nov-16 Dec-16 Jan-17 Feb-17 Mar-17 Apr-17 May-17 Jun-17 Jul-17 Aug-17 Sep-17 Oct-17 Nov-17 Earth Tech Environmental, LLC 11 Agenda Item #7 Page 12 of 20 2022 Clam Bay Water quality An@lysls Earth Tech Environmental, LLC www.e[eFlorida.com 12 Agenda Item #7 Page 13 of 20 2022 Clam Bay Water quality Analysis Sep-21 Oct-21 Nov-21 Dec-21 Jan-22 0.724 0.748 0.596 0.687 0.729 0.704 0.539 0.533 0.668 0.512 Feb-22 1.03 0.706 0.78/ 0.815 0.868 0.851 i..07. 0.809 Mar-22 1.22 1.05 1.11 1.12 1 1.06 3.32 0.922 0.973 Apr-22 0.937 0.973 0.691 0.639 0.782 0.975 1.05 0.926 May-22 1.08 0.974 0.833 0.811 0.707 0.756 0.825 0.8 Jun-22 1.1 0.688 0.645 0.616 0.749 0.739 0.685 0.587 Jul-22 0.928 0.666 0.624 0.675 0.554 0.584 0,543 0.565 0.547 Aug-22 1.33 0.865 1.07 0.67 0.797 0.826 0.922 0.88 0.847 Sep-22 1.05 0.81 0.688 0.854 0.673 0.746 0.839 1.19 0.812 Oct-22 19 1.11 1.06 1.64 0.799 0.845 0.9 0.771 0.959 Nov-22 Dec-22 0.895 0.876 0 •-6 Figure 4. Total Nitrogen concentrations per sample location over the period of January 2022 through December 2022 in Clam Bay, z 1.8 1.6 1.4 E 1.2 c w 0 1 z 0.8 F 0.6 0.4 0.2 0 1 Total Nitrogen (TN) 2 3 4 5 6 7 8 9 10 11 12 Month +CB1 CB2 CB3 CB4 t CBS -�-CB6 �-CB7 f CB8 -1-C89 Earth Tech Environmental, LLC 13 Agenda Item #7 Page 14 of 20 2022 Clam Bay Water quality Analysis 6.2 Dissolved Oxygen Saturation Dissolved Oxygen Saturation is out of compliance in 24/108 (22%) samples collected. DO dropped across most of the sample sites in January -August 2022, with slight increases in July, and increased during the last few months of the year. DO is measure of how much oxygen is dissolved in the water and consequently the amount of oxygen available to living aquatic organisms. Dissolved Oxygen Saturation is one of the most vital indicators of water quality as it determines not only the quantity of species, but diversity of species able to thrive. Human sources of oxygen -depleting organic materials include stormwater runoff, and/or failing septic systems (NOAA). The following tables and figures depict findings for compliance of DO at each sample location. Table 3. Dissolved Oxygen Saturation values per sample location over the period of January 2021 to December 2022 in Clam Bay in relation to the established standard criteria for Class II waters (42% saturation). Green represents samples in compliance with criteria. Red cells are below the established standard criteria. TABLE 3. Dissolved Oxygen Saturation Date Station Location (month/year) CB1 C62 CB3 CB4 CBS CB6 CB7 CB8 CB9 Jan-21 ..4 61 57.8 80.1-. 98.4 90.9 50 lan-21 61.4 80.4 76.8 83.784 82.6 84.9 Feb-21 60.9 76.2 79.3 87 89.1 91.9 80 Mar-21 40.1 51.8 74 81.1 83.1 77.9 71.4 Apr-21 40 32.8 62..5 63.1 76.3 90.3 96.7 93.7 ay-21 43.9 83.1 94.5 84.2 91.1 81.4 ne-21 .3 337 66.7 53.6 74.6 85.8 77.9 92.9 ly-21 j 10 21.6 42.6 63.2 75 69.3 72.4 g-21 6.3 26.6 40.3 27.2 44.5 789 887 74.5 71.9 53.8 p-21 29.6 61.1 782 78.8 Oct-21 26 52.2 57.2 75.1 91.9 88.3 73.6 Nov-21 6 51.2 55.4 62.7 105.4 98.8 108.3 97.3 78.3 Dec-21 44.2 56.8 61.6 71.7 85 85.2 Jan-22 6 $6.4 58.9 61..7 86.9 96.6 94.7 89.8 Feb-22 44.6 65.4 75.9 76.2 94 97 11L6 Mar-22 2 25.9 69.3 38.1 52.2 90.7 92 80.9 Apr-22 50.6 65.9 85.9 78.6 93.6 95.2 89 May-22 26 57 51.5 64.1 84.1 85.4 78.4 Jun-22 20.4 34.5 46.1 66.3 73.5 78.1 74.9 Jul-22 40.9 60.1 67.5 75.6 65 76.2 82.6 Aug-22 23.8 64.4 62.7 77.8 80 72.7 70.9 CY Sep-22 34.7 8.6 56.1 53.2 67.3 95.5 79.4 55.6 85.8 72.8 72.4 Oct-22 17.6 28.7 72.3 82.9 Nov-22 -5 Dec-22 129 42.7 23.8 55.5 1 55.1 69.5 82.7 98.2 93.5 119.2 85.9 107.4 f Earth Tech Environmental, LLC 14 www.eteflorida.com Agenda Item #7 Page 15 of 20 2022 Clam Bay Water Quality Analysis Figure 5. Dissolved Oxygen Saturation values per sample location over the period of January 2022 through December 2022 in Clam Bay, Dissolved Oxygen (DO) Lower Limit: 42% 140 120 e � 100 o_ 80 v 60 c m 40 x O 20 0 i � 1 2 3 4 5 6 7 8 9 10 11 12 Month t CB1 �CB2 J. --CB3 CB4 +CB5 t C66 tC67 tC88 —�—C89 Limit Earth Tech Environmental, LLC www.eteflorlda.com 15 Agenda Item #7 Page 16 of 20 2022 Clam Bay Water Quality Analysis 6.4 Copper Copper is out of compliance in 5/108 (0.05%) samples collected, primarily at CB1 from June to September 2022. Chronic Copper above 3.7 ug/L exposure can adversely affect fish health including damage to gills, liver, kidney, immune system, and nervous system. Prolonged copper exposure also may result in reduced fish growth and eventually death (Wong et al. 2018). According to NOAA, natural sources of Copper In aquatic systems Include weathering and erosion of rocks and soils. Anthropogenic sources of Copper include pesticide use and algaecides. A majorsource of Copper in the marine environment is antifouling paints, used as coatings for ship hulls, buoys, and underwater surfaces, and as a contaminant from decking and pilings. Table 4. Copper values per sample location over the period of January 2021 through December 2022 in Clam Bay in relation to the established standard criteria for Class II waters (3.7 ug/ Q. Green represents samples in compliance with criteria. Red cells are above the established standard criteria. TABLE 4. Copper (µg/L) Sample Location Date CB1 (month/year) CB2 CB3 CB4 CBS CB6 CB7 CB8 CB9 Jan-21 29 Feb-21 3.5 9- 2.1.3 -�.. ... - 2 3 1.2 151 -. 3.05 1.82 1.2 1..2 1.32 Mar-21 2.43 6.38 2.24 1.2 L2 1.2 1.2 1.2 Apr-21 .89 3.85 3.02 2.12 1.53 1.81 2.11 3.1 May-21 6.58 5.3 3.69 12 L2 2.05 Z57 254 June-21 3.04 2.79 2.18 1.75 1.67 1..47 2.03 1.65 July-21 2.05 2.35 1.65 1.54 1.13 1.2 1.94 1.05 Aug-21 2.5 1.89 1.4 1.35 1.18 1.05 1.17 1.05 Sep-21 2.38 1.41 L27 0.98 1.05 1.05 1.05 1.05 Oct-21 1.17 1.05 1.05 1.05 1.2 1.35 1.2 1.2 Nov-21 1.59 2.1 1.43 1.45 1.05 1.05 1.16 1.1 Dec-21 1.37 227 2A6 1.44 1.2 12 2.5 1.2 - Jan-22 2,44 2.68 2.38 2.51 1.2 1.41 1.2 L2 Feb-22 -: 2.37 2.91 2.31 1.93 12 1.32 1.36 L2 Mar-22 45. 16 3.18 5.02 1.9 1.05 2.44 2.21 128 Apr-22 " 2.19 1.72 2.17 1.2 1.2 1.4 1.73 1.2 May-22 2.04 2.91 1.83 1.96 L2 1.2 1.2 1.2 Jun-22 3.16 2.52 1.81 1.49 1.2 1.2 1.2 L2 Jul-22 .17 2.96 2.48 3.38 1.05 1.2 1.2 1.2 Aug-22 .27 2.85 2.05 1.27 1.05 1.2 1.72 1.2 Sep-22 .92 2.85 1.11 1.05 1.2 1.05 1.u5 1.05 Oct-22 1.26 2.44 1.2 1.2 1.05 1.2 1.2 1.2 Nov-22 0.9 L14 LOS 1.05 1.2 1.2 L21 1.29 Dec-22 - 1.05 1.18 1.2 1.2 1.2 1.2 1.2 Earth Tech Environmental, LLC 16 www.eteflorida.com Agenda Item #7 Page 17 of 20 2022 Clam Bay Water Quality Analysis Figure 6. Copper saturation values per sample location over the period of January 2022 through December 2022 in Clam Bay. 1a 9 8 7 6 v 5 a 0 4 V 3 2 1 0 1 Copper(Cu) Upper Limit: 3.7 ug/L 2 3 4 S 6 7 8 9 10 11 12 Month tcB1 �•CB2 9—CB3 —�—CB4 —HCBS #—CB6 tC87 �—CB8 t CB9 Limit Earth Tech Environmental, LLC www.eteflorida.com 17 Agenda Item #7 Page 18 of 20 2022 Clam Bay Water quality Analysis 6.5 Chlorophyll -a Chlorophyll -a is out of compliance for 36/107 (36%) of samples collected, making Clam Bay impaired for Chlorophyll -a. Table 5. Chlorophyll -a values per sample location over the period of January 2022 through December 2022 in Clam Bay. Highlighted values fall below the established standard criteria for Class II waters (11 ug/L). Green represents samples in compliance with criteria. Red cells indicate exceedance of criteria. Figure 7. Chlorophyll -a values per sample location over the period of January 2022 through December 2022 in Clam Bay, 50 45 40 35 00 30 S 25 n 0 20 `o 15 10 , 5 1 0 Chlorophyll -a Upper Limit: 11 ug/L 1 2 3 4 5 6 7 8 9 10 11 12 Month —O—CB2 CB3 CB4 t CB5 �—CB6 —C67 —f—CB8 t CB9 Limit Environmental, LLC www.eteflorida.com 18 Agenda Item #7 Page 19 of 20 2022 Clam Bay Water quality Analysis The following analysis shows that Total Phosphorus has a direct correlation on increases in Chlorophyll -a and Total Nitrogen, triggering these analytes to also increase. Total Phosphorus also has an inverse correlation on Dissolved Oxygen Saturation; reducing the amount of oxygen, increasing eutrophication, dead zones (hypoxia) and decreasing pH (ocean acidification). Figure 8. Direct relationship between Total Phosphorus and Total Nitrogen over the period of January 2022 through December 2022 in Clam Bay. Total Phosphorous vs. Total Nigrogen z 1.8 • 1.6 • R2 = 0.5463••• m • • ...*... 1.2 • • • ....�.....• • 0 1 z 0.8 • % �'�♦.•�....•• • 00 ... 0.4 0.2 0 -- 0 0.05 0.1 0.15 0.2 0,25 0.3 Total Phosphorous (mg/L) Figure 9. Inverse relationship between Total Phosphorus and Dissolved Oxygen Saturation over the period of January 2022 through December 2022 in Clam Bay, Total Phosphorous vs. Dissolved Oxygen Saturation 120 0 100 • • /• • • m 80 • , �• �• • • ,m 60 , •..r..+.. • 40 Ox • "••.......,• v 20 • •� M••• • • o • • : .............•• o 0.05 0;1 0,15 0.2 0.25 '•' -20 Total Phosphorous (mg/L.) 0.3 Tech Environmental, LLC 19 Agenda Item #7 Page 20 of 20 2022 Clam Bay Water Quality Analysis Figure 10. Direct relationship between Total Phosphorus and Chlorophyll -a over the period of January 2022 through December 2022 in Clam Bay. Total Phosphorous vs. Chlorophyll -a 50 45 • 40 • 'Q 35 • ; 30 •• • R2=0.29.17..•• a 25 • • 0 20 • • • • 15 �• 1,f.............. U • 10 • •N S� • ••. 5 001 •• • 4i••i • 0 0.05 0.1 0.15 0.2 0.25 Total Phosphorous (mg/L) 7.0 DISCUSSION &RECOMMENDATIONS 0.3 Total Phosphorus Total Phosphorus levels exceeded the NNC in 43/107 (40%) of samples collected. Data and prior reports show that Total Phosphorus levels have been consistently out of compliance over the last eight years. Total Nitrogen Total Nitrogen levels exceeded the NNC in 17/107 (1676) of samples collected. Dissolved Oxygen Saturation Dissolved Oxygen Saturation levels exceeded the NNC in 24/108 (22%) of samples collected. Copper Copper is out of compliance in 5/108 (0.05%) of samples collected. Chlorophyll -a Chlorophyll -a levels exceeded the NNC in 36/108 (33%) of samples collected. Clam Bay experienced a few major disturbances in 2022 including the dredging of Clam Pass (February through April 2022) and Hurricane Ian on September 28, 2022, which brought 8-10 feet of storm surge into Clam Bay. These events potentially contributed to increased nutrient loads and decreased dissolved oxygen levels by stirring up sediments. These major disturbances make it difficult to pinpoint definitive causes of exceedances, especially for Total Phosphorus and Total Nitrogen. Additional testing including sediment sampling or comparisons to berm outfalls may be beneficial to more acutely pinpoint potential causes and duration of elevated nutrient levels and decreased Dissolved Oxygen. LLC www.eteflorida.com 20