Agenda 04/11/2023 Item #14B ( Pilot test program with Ecological Laboratories of the Cape Coral Florida)14.B.1
04/ 11 /2023
EXECUTIVE SUMMARY
Recommendation that the Board of County Commissioners approve a pilot test program with Ecological
Laboratories of Cape Coral Florida to monitor and document the result of using Ecological Laboratories
product "Quantum Growth Series" a soil and plant microbial inoculant within the existing landscape right
of way within the Bayshore Gateway Triangle Community Redevelopment Area (BGTCRA) and accept the
donation of the product.
OBJECTIVE: The goal of this pilot test program is to determine if municipalities can reduce reliance on nitrogen -
based fertilizers by using a bacterial inoculant (Quantum product) alone or in combination with reduced amounts of
fertilizers. The pilot test program will compare the different treatment plans. The pilot test program protocol results
in the collection of visual and plant tissue data to determine the success of the bacterial inoculant product. The pilot
test program will be conducted outdoors and not in a controlled environment.
CONSIDERATIONS: On June 13, 2022, Aaron Sane, Director, Crop Agriculture, of Ecological Laboratories (EL)
provided a presentation of their product Quantum Growth Series to the BGTCRA Advisory Board (Exhibit A). A
similar presentation was also provided to the Bayshore Beautification MSTU Advisory Committee on July 13,
2022. At both meetings, Mr. Sane described the benefits of the product, not only to the plants, but also the
environment.
Ecological Laboratories reports that Quantum Growth series primary benefits are:
• Improve plant photosynthesis
• Improve nutrient availability transfer
• Enhance / restore soil biology
• Improved root structure
• Increased shoot growth
• Improved plant health
• Increased yield
• Enhance/Restore
• Soil Biology
Several County staff have reviewed the products and supporting materials, as well as, several private sector
professionals, including staff at Naples Botanical Garden and Hamutahl Cohen, Ph.D. University of Florida
Extension, collectively referred to as "team members." All recommend and have agreed a six-month pilot test
program of the product would be beneficial to determine the next steps. The proposed pilot test program protocol
was developed and approved by team members. The pilot test program's boundary and the protocols are outlined in
Exhibit B. The team members are assigned the following tasks:
• BGTCRA Project Manager II will be responsible for managing the project and collecting monthly
photographs.
• Beautification MSTU Landscape Maintenance Contractor (Contract #21-7846R) will be applying the
products as part of the normal maintenance contract.
• Dr. Cohen, University of Florida Extension, will be responsible for reviewing the data and providing a
summary at the conclusion of the study at no charge. (Exhibit C)
• Ecological Laboratories provided a soil analysis in November 2022 (pre -study analysis Exhibit D) and will
be donating the product, valued at approximately $60. No further soil analysis is required. (Exhibit E).
• BGTCRA Project Manager II will provide monthly status reports to the team members and Advisory
Boards.
The results of the final study will be presented to the Board of County Commissioners for further direction and
consideration. The"Quantum Growth Series" product is considered a sole source proprietary technology and the
County is not obligated to make future purchases. Should the Board of County Commissioners entertain future
purchases, Staff will seek a waiver of competition.
Packet Pg. 109
14.B.1
04/ 11 /2023
GROWTH MANAGEMENT IMPACT: No impact at this time.
ADVISORY BOARD RECOMMENDATION: The BGTCRA Advisory Board voted 5-2 to support a pilot
study. Bayshore Beautification MSTU Advisory Committee voted 6-0 to support a pilot study. The Advisory
Boards reviewed the final pilot test program boundaries and the protocols at their December 6 and December 14
meetings, respectively.
LEGAL CONSIDERATIONS: This item is approved for form and legality, and requires a majority vote for
Board approval. (HFAC)
FISCAL IMPACT: Ecological Laboratories will donate the material for the pilot test program. Funding for the
existing landscape maintenance contractor is provided within Bayshore Beautification MSTU Fund (163).
RECOMMENDATION: The Board of County Commissioners approve a study with Ecological Laboratories of
Cape Coral Florida to monitor and document the result of using Ecological Laboratories product "Quantum Growth
Series" a soil and plant microbial inoculant within the existing landscape right of way within the Bayshore Gateway
Triangle Community Redevelopment Area (BGTCRA) and accept the donation of the product.
Prepared by: Tami Scott, Project Manager
ATTACHMENT(S)
1. Exhibit A- Presentation QG Benefits Overview - 6.8.22 (PDF)
2. Exhibit B- pilot test program DesignProtocol_final (DOCX)
3. Exhibit C-Dr Cohen (PDF)
4. Exhibit D-MTSU Trial Soil Fertility Analysis 11.11.22 (PDF)
5. Exhibit E- ELI LOU (PDF)
6. Exhibit F- Sole SourceELI LOU2 (PDF)
Packet Pg. 110
14.B.1
04/ 11 /2023
COLLIER COUNTY
Board of County Commissioners
Item Number: 14.B.1
Doc ID: 25023
Item Summary: Recommendation that the Board of County Commissioners approve a pilot test program with
Ecological Laboratories of Cape Coral Florida to monitor and document the result of using Ecological Laboratories
product " Quantum Growth Series" a soil and plant microbial moculant within the existing landscape right of way
within the Bayshore Gateway Triangle Community Redevelopment Area (BGTCRA) and accept the donation of
the product. (Debrah Forester, Community Redevelopment Agency Division Director)
Meeting Date: 04/11/2023
Prepared by:
Title: — Bayshore CRA
Name: Tami Scott
03/21/2023 9:27 AM
Submitted by:
Title: CRA Division Director — Corporate Business Operations
Name: Debrah Forester
03/21/2023 9:27 AM
Approved By:
Review:
Corporate Business Operations
Jennifer Reynolds
Stage 1 Review
Corporate Business Operations
Geoffrey Willig
Stage 1 Review
Corporate Business Operations
Debrah Forester
Director - CRAs
County Attorney's Office
Heidi Ashton-Cicko Level 2 Attorney of Record Review
Procurement Services
Sandra Herrera
Additional Reviewer
Office of Management and Budget
Debra Windsor
Level 3 OMB Gatekeeper Review
County Attorney's Office
Jeffrey A. Klatzkow Level 3 County Attorney's Office Review
Corporate Compliance and Continuous Improvement
Megan Gaillard
Office of Management and Budget
Christopher Johnson Additional Reviewer
County Manager's Office
Geoffrey Willig
Level 4 County Manager Review
Board of County Commissioners
Geoffrey Willig
Meeting Pending
Completed
03/30/2023 4:58 PM
Skipped
03/30/2023 5:01 PM
Completed
03/31/2023 8:54 AM
Completed
03/31/2023 10:14 AM
Completed
03/31/2023 12:31 PM
Completed
03/31/2023 2:30 PM
Completed
03/31/2023 3:36 PM
Additional Reviewer
Completed
04/01/2023 7:40 PM
Completed
04/03/2023 8:14 AM
Completed
04/03/2023 11:54 AM
04/11 /2023 9:00 AM
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14.B.1.b
"Exhibit B "
Quantum Growth Series -microbial inoculants
Pilot Project Bayshore BGTCRA and MSTU
Study Goals:
The goal of this study is to determine if municipalities can reduce reliance on nitrogen -
based fertilizers by using a bacterial inoculant (Quantum product) alone or in combination
with reduced amounts of fertilizers. The study design compares different treatment
plans. The study protocol results in the collection of visual and plant tissue data to
determine the success of the bacterial inoculant product.
Study Design:
There are 4 study areas. Study areas A, B, and C contain perennial peanut. Each area will
each be divided into 4 treatments.
Treatment
Area A
Area B
Area D
C: Control (no Quantum
Al
131
D1
application, regular fertilizer
schedule)
Q: Quantum Product only
A2
B2
D2
QR: Quantum Product +
A3
B3
D3
reduced amount of fertilizer
QF: Quantum Product +
A4
B4
D4
full amount of fertilizer
Study Area C only contains bougainvillea. It is too small for 4 distinct treatments. It will
therefore be divided into 2 sections, split down the middle of the bougainvillea bed.
Treatment
Area C
C: Control (no Quantum
C1
application, regular fertilizer
schedule)
Q: Quantum Product only
C2
See photos for images of each study site. These are available in large-scale image format
in a separate document.
Packet Pg. 123
14.B.1.b
Study area "A" — a -
four sections of o
peanut
Study area " D"
four sections of median
.ice d
s
Study Protocol & Timeline
The study duration will be 6 months. During this time, treatments receiving fertilizer
treatments and quantum treatments will receive applications (C, Q, QR, QF) at the
recommended intervals and dosages for the periods. Applications should follow label
regulations and recommendations from Quantum products and the fertilizer company
supplying the fertilizer product. The study period will begin on the day of the first
application.
Equipment:
Quantum inoculant, fertilizer, stake flag., quadrant made of PVC pipe or string, sharpie,
camera, computer, zip locks
Repeat the following protocol every 30 days, for a max of 6 times.
- At each treatment area (Al-A4, 131-134, etc.), please a stake flag. Label the flag with the
Site and Treatment (e.g., Al). This flag will stay in place for the duration of the entire
project period and does not need to be replaced every 30 days unless it is damaged.
Every 30 days, check to make sure label has not rubbed off.
- Collect 2 photos of each treatment within each area (Al-A4, 131-134, etc.). The first
photo should be a landscape photo of the median, taken at a safe distance,
approximately 15 feet from each median. The second photo should be taken as a bird
Packet Pg. 124
14.B.1.b
eye's view a 3-foot square quadrant within each median. Use 3ft PVC sticks or string
to mark out the area. The flag should be at the center of the quadrant so that photos
are always taken in the exact same spot during each sample period. Take photo so
that the entire quadrant is in the photo, ideally from chest -height and directly above
the quadrant looking down.
- Upon returning home, immediately upload the photos into a folder on your computer
and label each file with the site, treatment, date, and photo type (e.g., A2_Feb-2-
23_median and A2_Feb-2-23_quadrant)
Repeat the following protocol on the first day and last day of the project (optional
protocol)
- Collect leaf sample of perennial peanut at the center of each site treatment for A,
B, and D.
- Place each sample in its own Ziplock bag
- Submit to OF/IFAS for tissue analysis or to another laboratory. E.g.,
https://soiIslab.ifas.ufLedu/ESTL%20Tests.asp
- Make sure you label the samples with the date, site, treatment (e.g., A2_Feb-2-23)
and mark this on your submission form as well
- To learn more about tissue samples, how to collect tissue samples, and where to
submit, see this link: https://edis.ifas.ufl.edu/publication/EP081
Analyzing the data:
- At the completion of the study period, each quadrant photo and median photo
will be ranked with a rating system (1-5) to evaluate:
o vegetation color
o vegetation density
o vegetation health/vigor
If quantum alone or quantum + reduced fertilizer treatments provide results equal
to or better than quantum + full fertilizer, we can determine that using a bacterial
inoculant quantum product can be applied. This will reduce the need for fertilizer
in the region.
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WAT IRS IC L RAIL Soil Analysis
LA OR IE NC.
Waters Agricultural Laboratories, Inc WAScWe�'
257 Newton Hwy I Camilla, GA 31730- 1 Phone (229) 336-7216
ECOLOGICAL LABORATORIES Grower: CITY OF NAPLES Received: 11/14/2022
Farm ID: CITY OF NAPLES Processed: 11/16/2022
2525 N E 9TH AVE Field ID:
CAPE CORAL, FL 33909 Lab Number: 791212KC
UNITED STATES Layer ID:
Test Method: Mehlich
I
Soil
Laboratory
Data (lbs/a)
Target pH
6.5
P K
Mg
Ca
Soil pH
Buffer pH
S
B
Zn
Mn
Fe
Cu
Phosphorus Potassium
Magnesium
Calcium
Adams -Evans
Sulfur
Boron
Zinc
Manganese
Iron
Copper
20 L
31 L
306 VH
9573 VH
8.1
7.90
16 L
0.7 M
1.6 L
6 L
10 M
0.2 L
Al
Na
NO3-N
NH4
Cl
Soluble
Organic
ENR
Mo
Ni
BiCarbs
Salts
Matter
Aluminum
Sodium
Nitrate-N
Ammonia
Chloride
Molybdenum
Nickel
0.76
15
ppm
ppm
mmhos/cm
%
ppm
ppm
meq/L
Very H
High
Adequa
Mediul
Lo,
Soil Analysis Ratings
P K Mg Ca S B Zn Mn Fe Cu
Cation "
Exchange 26.0 meq/100g o
Capacity
u
c
Base Saturation '
K: 0.2 % Mg: 4.9 % T
IV
Ca: 91.9 %
H: 3.1 %
L
Na: %
i
Base Saturation
❑ %K 2
D%Mg (
u
❑ %C a
D %H U
i
■ %N a i
F
Lime Gypsum N P205 K20 Mg S B Zn Mn Fe Cu
Nitrogen Phosphate Potash Magnesium Sulfur Boron Zinc Manganese Iron Copper J
J
2.3 2.1 0.3 0.01 0.02 0.23 0.02 I:
* = Maintenance Recommendation
APPLY .2 LBS OF NITROGEN PER TREE-1/2 IN EARLY MARCH-1/2 IN JUNE.
1
This document may be reproduced only in its entirety. Waters Agricutural Laboratories has no control over the manner in which samples are taken, therefore, analysis is based solely on the sample as received. Labor
assessed on the referenced sample. Packet Pg. 127
WAT IRS IC L RAL
LA OR IE , NC.
ECOLOGICAL LABORATORIES
2525 N E 9TH AVE
CAPE CORAL, FL 33909
UNITED STATES
Soil Analysis
Waters Agricultural Laboratories, Inc
257 Newton Hwy I Camilla, GA 31730- 1 Phone (229) 336-7216
Grower: CITY OF NAPLES
Farm ID: CITY OF NAPLES
Field ID:
Lab Number: 791213KC
Layer ID:
14.B.1.d
WASCW' I ltiL/'
Received: 11 /14/2022
Processed: 11 /16/2022
Test Method: Mehlich
I
Soil
Laboratory
Data (lbs/a)
Target phl
6.5
P K
Mg
Ca
Soil pH
Buffer pH
S
B
Zn
Mn
Fe
Cu
Phosphorus Potassium
Magnesium
Calcium
Adams -Evans
Sulfur
Boron
Zinc
Manganese
Iron
Copper
8 L
30 L
263 VH
9950 VH
8.4
7.95
12 L
0.4 L
1.6 L
2 L
6 L
0.2 L
Al
Na
NO3-N
NH4
Cl
Soluble
Organic
ENR
Mo
Ni
BiCarbs
Salts
Matter
Aluminum
Sodium
Nitrate-N
Ammonia
Chloride
Molybdenum
Nickel
1.21
24
ppm
ppm
mmhos/cm
%
ppm
ppm
meq/L
Very H
High
Adequa
Mediul
Lo,
Soil Analysis Ratings
P K Mg Ca S B Zn Mn Fe Cu
Cation
Exchange 26.4 meq/100g Ih
Capacity p
Ili
C4
Base Saturation
N
K: 0.1 % Mg: 4.1 % r
Ca: 94.2 % H: 1.5 %
rn
Na: % >
Base Saturation Q
❑ %K
D %M g
❑ %C a ;
D %H 1
■ %N a
I
• •: TURF GRASS SOD
Fertility Recommendations•
1 1 1
•Yield:
Lime Gypsum N
P205
K20
Mg S
B Zn
Mn Fe Cu
Nitrogen
Phosphate
Potash
Magnesium Sulfur
Boron Zinc
Manganese Iron Copper
2.9
5.1
0.5
0.01 0.02
0.23
0.1 0.02
5.3
' = Maintenance Recommendation
NITROGEN AND POTASSIUM SHOULD BE APPLIED IN SPLIT APPLICATIONS - PREFERABLY 3 TO 5 TIMES - PREDICATED ON THE TYPE OF
NITROGEN USED. PLANT SAMPLES (GRASS CLIPPINGS) SHOULD BE TAKEN DURING THE GROWING SEASON TO MONITOR ALL NUTRIENTS.
ADDITIONAL NUTRIENTS MAY BE NEEDED ESPECIALLY NITROGEN AND POTASSIUM.
This document may be reproduced only in its entirety. Waters Agricutural Laboratories has no control over the manner in which samples are taken, therefore, analysis is based solely on the sample as received. Labor
assessed on the referenced sample. Packet Pg. 128
WAT IRS IC L RAL
LA OR IE , NC.
ECOLOGICAL LABORATORIES
2525 N E 9TH AVE
CAPE CORAL, FL 33909
UNITED STATES
Soil Analysis
Waters Agricultural Laboratories, Inc
257 Newton Hwy I Camilla, GA 31730- 1 Phone (229) 336-7216
Grower: CITY OF NAPLES
Farm ID: CITY OF NAPLES
Field ID:
Lab Number: 791216KC
Layer ID:
14.B.1.d
WASCW' I ltiL/'
Received: 11 /14/2022
Processed: 11 /16/2022
Test Method: Mehlich
I
Soil
Laboratory
Data (lbs/a)
Target pH
6.5
P K
Mg
Ca
Soil pH
Buffer pH
S
B
Zn
Mn
Fe
Cu
Phosphorus Potassium
Magnesium
Calcium
Adams -Evans
Sulfur
Boron
Zinc
Manganese
Iron
Copper
25 L
53 L
351 VH
9691 VH
8.1
7.90
15 L
1.1 A
1.8 L
4 L
6 L
0.2 L
Al
Na
NO3-N
NH4
Cl
Soluble
Organic
ENR
Mo
Ni
BiCarbs
Salts
Matter
Aluminum
Sodium
Nitrate-N
Ammonia
Chloride
Molybdenum
Nickel
1.5
30
ppm
ppm
mmhos/cm
%
ppm
ppm
meq/L
Very H
High
Adequa
Mediul
Lo,
Soil Analysis Ratings
P K Mg Ca S B Zn Mn Fe Cu
Cation
Exchange 26.6 meq/100g Ih
Capacity p
Ili
i:V
Base Saturation
N
K: 0.3 % Mg: 5.5 % r
7
Ca: 91.2 %
H: 3.0 %
rn
Na: %
>
Base Saturation a
❑ %K
D %M g 1r
O
LL
❑ %C a
O
D %H N
■%Na '�
H
• • ORNAMENTAL
Fertility Recommendations•
1 1 1
•Yield:
Lime
Gypsum N
P205
K20
Mg S
B Zn
Mn
Fe
Cu
Nitrogen
Phosphate
3.6
Potash
5.1
Magnesium Sulfur
0.3
Boron Zinc
0.02
Manganese
0.23
Iron
0.1
Copper
0.02
2.8
' = Maintenance Recommendation
SPLIT APPLICATIONS OF NITROGEN AND POTASSIUM RECOMMENDED. PLANT SAMPLES SHOULD BE TAKEN DURING THE GROWING
SEASON. ADDITIONAL OR SUPPLEMENTAL NUTRIENTS MAY BE NEEDED.
This document may be reproduced only in its entirety. Waters Agricutural Laboratories has no control over the manner in which samples are taken, therefore, analysis is based solely on the sample as received. Labor
assessed on the referenced sample. Packet Pg. 129
February 16, 2023
RE: Thomasson Drive Fertilizer Study
To whom it may concern.
"Exhibit E "
With this letter, Ecological Laboratories Inc (ELI) is affirming the following
statements:
1. Ecological Laboratories is donating the bacterial inoculant (Quantum -Total) for the 6
month trial.
2. Ecological Laboratories is donating time of key personnel to evaluate the trial.
3. The approximate cost of the Ecological Laboratories donated material (1-2 gals of
Quantum -Total) is $30-60 total.
4. Ecological Laboratories understands that regardless of the outcome of the trial, Collier
County has no obligation to purchase ELI products.
Sincerely,
Aaron Sane
Director of Agriculture, Horticulture and Turf
Ecological Laboratories, Inc.
I
rM
Packet Pg. 130
March 28, 2023
RE: Quantum -Total product
To whom it may concern.
With this letter, Ecological Laboratories Inc. is affirming that we are the sole
manufacturer of the Quantum -Total product and that its composition is unique and
proprietary in nature. While this product may be acquired thru our distribution network,
we are the sole manufacturer of the product and are providing this product at no charge
for evaluation and at significant discount ($16 per gallon) if adopted as an input.
Sincerely,
Aaron Sane
Director of Agriculture, Horticulture and Turf
Ecological Laboratories, Inc.
Packet Pg. 131