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Agenda 12/10/2014 PELICAN BAY SERVICES DIVISION Municipal Services Taxing & Benefit Unit NOTICE OF PUBLIC MEETING DECEMBER 10, 2014 THE WATER MANAGEMENT COMMITTEE OF PELICAN BAY SERVICES DIVISION WILL MEET AT 1:00 PM ON WEDNESDAY, DECEMBER 10 AT THE COMMUNITY CENTER AT PELICAN BAY, LOCATED AT 8960 HAMMOCK OAK DRIVE, NAPLES, FL 34108. AGENDA 1 . Roll call 2. Agenda approval 3. Audience comments 4. Update on pilot lake treatments, including status of blue tilapia installations 5. Discussion of lake bank erosion restoration methods 6. Update on copper levels in lakes and Clam Bay 7. Update on filtration systems: filter marsh vs. vacuum airlift 8. Developing an educational outreach campaign to alert residents as to the presence of nutrients in recycled water and the need to reduce use of fertilizer as a result 9. Adjournment ANY PERSON WISHING TO SPEAK ON AN AGENDA ITEM WILL RECEIVE UP TO ONE (1) MINUTE PER ITEM TO ADDRESS THE BOARD. THE BOARD WILL SOLICIT PUBLIC COMMENTS ON SUBJECTS NOT ON THIS AGENDA AND ANY PERSON WISHING TO SPEAK WILL RECEIVE UP TO THREE (3) MINUTES. THE BOARD ENCOURAGES YOU TO SUBMIT YOUR COMMENTS IN WRITING IN ADVANCE OF THE MEETING. ANY PERSON WHO DECIDES TO APPEAL A DECISION OF THIS BOARD WILL NEED A RECORD OF THE PROCEEDING PERTAINING THERETO, AND THEREFORE MAY NEED TO ENSURE THAT A VERBATIM RECORD IS MADE, WHICH INCLUDES THE TESTIMONY AND EVIDENCE UPON WHICH THE APPEAL IS TO BE BASED. IF YOU ARE A PERSON WITH A DISABILITY WHO NEEDS AN ACCOMMODATION IN ORDER TO PARTICIPATE IN THIS MEETING YOU ARE ENTITLED TO THE PROVISION OF CERTAIN ASSISTANCE. PLEASE CONTACT THE PELICAN BAY SERVICES DIVISION AT (239) 597-1749 OR VISIT PELICANBAYSERVICESDIVISION.NET. 12/8/2014 2:51:42 PM ResnickLisa Subject: Lake Bank Erosion Methods From: Jim Carr [mailto:jim.carr@abbinc.com] Sent: Tuesday, December 02, 2014 4:32 PM To: ResnickLisa Cc: Kevin Carter; Neil Dorrill Subject: FW: North Berm + Riprap & Riprap vs Geo-textile Tubing? Hi Lisa, I am out of town but I asked Tom Barber to answer your Lake Bank erosion questions. Tom and Rick Barber have worked on some lake bank restoration projects recently in communities similar to Pelican Bay. See responses in red below. Thanks. Jim From:Tom Barber Sent: Monday, December 1, 2014 4:14 PM To:Jim Carr; Rick Barber Subject: RE: North Berm + Riprap & Riprap vs Geo-textile Tubing? Jim, here is what I have . . . See below in red for question 3. Thanks, Tom Barber Agnoli, Barber, & Brundage Inc. 7400 Tamiami Tr. N.,Suite 200,Naples, FL 34108 Phone(239) 597-3111 barber.t @abbinc.com Lake Bank Erosion We are coordinating a lake bank erosion survey to determine which lake banks need to be repaired and from that priority list, we will create a two-year schedule of lake bank restoration repair projects to recommend to our board. Currently, our lake bank restoration program uses the geo-textile tubing method; however, we have been getting increased requests for riprap. 1. What is the cost per unit difference between riprap and geo-textile tubing? Rip Rap averages around $85 per linear foot installed, ranging from$55 to $120 depending on the products used and construction access. Geotextile tubing costs range from $40 to $60 per foot and averages around $50 per linear foot. 2. For lake bank erosion, is one method more effective? Our experience has lead us to believe that Geotubes are not the most robust long term solution, they exacerbate the lake bank grade differential problem and create an artificial wall that can break and lead to erosion over time. In dry months when lake levels are low the geotubes can be directly exposed to sunlight and the material they are comprised of can be susceptible to breakdown/cracking. We have recently replaced failing geotube that was installed no more than 5 years previously in other communities, the method we replaced it with is known as cellular confinement The cellular confinement (GeoWeb) method utilizes an anchored HDPE grid that has cells that are then filled with stone, picture shown below. The entire product is covered by dirt and sod, leaving no exposure to sunlight and a reinforced lake bank that recreates the gradual slope of the backyard into the lake and extends 2' below the control elevation of the lake. We can provide more information about this product and design examples of areas of successful lake bank remediation. 1. GeoWeb (during construction) j jay y. t jE "°7 ": ' w�" a, : �* °�+s' +Mtn' .. ' '� .... 17+i, .may„ t`Al t. ., N..+ rv,m' •9s'T��' Wit+ ' •--° ,... . - - — - rte"` ^k . ter-- "' m"l s':: '�r..;'-- -. .- -a..., ,,, -.- x' ....„ ,, jam, �' 4, , _ „,.., ..r.,..,_ _ r gielpofro010. *'' ,,- ' , tr.•f. ' ., . yi :fit r ut,5� !444 � I•' 'T e 4 S t? Also, the planting of heavy littoral plants and re-grading the lake bank can help stabilize the side slopes and greatly decrease the potential for future erosion. Pelican Bay is fortunate to be closer to the coast and have minimal lake fluctuation which lends itself to sustain the health of the littoral plantings through the dry season. 3. We understand that are the limitations to using riprap to repair lake bank erosion. What are those limitations? The first limitation is governmental, both Collier County and South Florida Water Management District regulate that you cannot exceed 40%of the lake's total perimeter in rip-rap and/or retaining wall. Physical limitations included limited access, if placed behind homes, the homeowners no longer have unobstructed access to the lake bank. In heavy wind events smaller rip-rap has the propensity to move in wave action and wear holes in the filter fabric below. This can lead to unwanted upland erosion in certain cases. Aesthetic limitations also exist, over time algae/mold can develop on the rip-rap. 2 ResnickLisa From: david trecker[djtrecker @yahoo.com] Sent: Thursday, December 04, 2014 1:23 PM To: ResnickLisa Subject: Lakebank Erosion Lisa - Please transmit to Neil, with a copy to Tom Cravens and Susan O'Brien. Neil - I have a number of questions about the lake bank erosion data you presented to the board yesterday. - Who measured it and when? - How were the drop-off measurements made? - Were the measurements made all around the indicated lakes or just at one or two spots? - Is there any indication the other 59 lakes have similar erosion problems? I'm concerned because this issue was dropped on us without any warning and because its remediation will obviously be costly. A response before the Water Management Committee meeting on Wednesday would be appreciated. Thanks, Dave 1 ResnickLisa Subject: Wed, Dec 10 Water Management Committee FW: Blue Tilapia From: david trecker[mailto:djtrecker(ayahoo.com] Sent: Saturday, December 06, 2014 11:37 AM To: ResnickLisa Subject: Fwd: Blue Tilapia Lisa-Please forward to the other PBSD directors and Neil and Marion. Also, please include in packet for the WMC meeting. Thanks,Dave Begin forwarded message: From: Mike Bauer<mbauer(a�naplesgov.com> Subject: FW: Blue Tilapia Date: December 5, 2014 at 2:37:50 PM EST To: "ditrecker(a vahoo.com" <ditrecker(c�yahoo.com> From: Katie Laakkonen Sent:Tuesday, May 27, 2014 2:48 PM To: Mike Bauer Subject: Blue Tilapia http://en.wikipedia.org/wiki/Oreochromis aureus Invasive species Since its introduction into Florida in 1961m the fish has increased its range and frequency of occurrence. It is now the most widespread foreign species in Florida, with established populations as far north as Lake Alice, in Gainesville. Florida.u It is a major management problem for the National Park Service due to its predominance in Taylor Slough in Everglades National Park, where it has changed the fish community structure.v The species is also expanding its range in Texas, is responsible for inhibition of the population of Largemouth Bass in Lake Trinidad, and is implicated in the unionid mussel declines in two bodies of water in Texas.0 It is also blamed for a severe decline in native fish populations in Warm Springs Natural Area.0 Katie Laakkonen Environmental Specialist City of Naples, Natural Resources 295 Riverside Circle Naples, FL 34102 Office:239-213-7122 Fax: 239-213-7127 klaakkonen@naplesgov.com www.naplesgov.com 1 ResnickLisa Subject: Wed, Dec 10 Water Management Committee From:david trecker [mailto:djtrecker @yahoo.com] Sent: Saturday, December 06,2014 11:40 AM To: ResnickLisa Subject: Fwd: Lisa-Please forward to the other directors and Neil and Marion. Also,please include in the packet for the WMC meeting. Thanks, Dave Begin forwarded message: From:Mike Bauer<mbauerRnaplesgov.com> Date: December 5,2014 at 2:37:33 PM EST To:"ditreckert vahoo.com"<ditrecker(a yahoo.com> U.S. Habitat: Fresh or brackish waters, ranging from creeks and streams to lakes.The blue tilapia is adaptable to salinity levels allowing it to inhabit and reproduce in both freshwater and brackish areas. http://www.tsusinvasives.oro/database/blue-tilapia.html Oreochromis aureus Blue Tilapia Class: Actinopterygii Order: Perciformes Family: Cichildae i i it �.1��'► ��'+t�-► Source:http://www.fishfarmingbusiness.com/ Description Adults range from about 5-8 inches in length and can weigh 5-6 pounds; however, the largest recorded specimen was up to 21 inches and weighed more than 10 pounds. Oreochromis aureus has a blue-grey body with a white belly and 20 to 26 gill rakers. The caudal fin of the blue tilapia has broad bright red or pink distal margin. The head of the male fish will change into a bright metallic blue shade, during the breeding season, and he will also display a vermilion pigmentation on the edge of his dorsal fin and an intense pink coloring on the margin of his caudal fin. A breeding female fish will develop a pale orange color on the edges of her dorsal and caudal fins. Ecological Threat: When Oreochromis aureus is present it can diminish plant, fish and shrimp diversity in freshwater areas. Blue tilapia has also been implicated as the cause for unionid mussel declines in two Texas water bodies, Tradinghouse Creek and Fairfield reservoirs. With a wide range of temperature toleration, the blue tilapia has been able to establish itself within the Southern Gulf States but could travel northwards. Oreochromis aureus is considered a competitor with native species for spawning areas, food, and space. Some streams where Oreochromis aureus is plentiful have lost most vegetation and nearly all native fishes. It has been shown in several states that the blue tilapia's local abundance and high densities in certain areas have resulted in marked changes in fish community structure. All species from the genus Oreochromis readily hybridize, potentially posing a threat to genetic diversity through introgression. If two species from the genus hybridize, that can increase their survivability and expand their invasive range. 1 TELEPHONE CONVERSATION WITH BILL MORRIS (12/2/14) Bill Morris heads a group responsible for water management in Pelican Pointe, a 20-year-old community of 1388 units in Sarasota County just north of Venice, FL. I contacted Morris at the recommendation of Bill Risen, a friend of Paul Johansen, a resident of the Breakwater in Pelican Bay. Pelican Pointe has 67 retention ponds (lakes) in three basins, the runoff from which goes into Hatchet Creek. 55 of the lakes catch runoff from well water, which is used for irrigation. Algae in those lakes is controlled by littoral plantings, which are required by Sarasota County. (Twelve specific plants are recommended.) Some chemical treatment is used when necessary. Units in the basin containing the remaining 12 lakes use recycled water, which, like ours, is loaded with nutrients (dissolved nitrogen and phosphorus from fertilizer). Those 12 lakes are called "the dirty dozen" because of continuing algae problems. Aeration, plantings and chemical treatment are used in an effort to control algae bloom. Duckweed is less of a problem. Blue tilapia were put in all 67 lakes but were said to have had no long-term effect on the algae. The fish were considered a nuisance because they ate shoots from the littoral plants. An environmental engineer recommended to Sarasota County that the fish be removed from all of the lakes. An outside firm is to net the fish and sell them for food. Morris invites the PBSD to visit, view their lakes and review their activities. Dave Trecker (12/3/14) TELEPHONE CONVERSATION WITH RAFAEL VASQUEZ- BURNEY (11/19/14) Rafael is a member of CH2M Hill, the consulting outfit the PBSD uses to sample, analyze and report on water quality in our upland lakes. In an earlier Water Management Committee meeting, Rafael advised against using blue tilapia to control algae. My telephone call was intended as a follow-up on those comments. Rafael said the tilapia gills catch the macro algae but let the small algae pass through. The small algae thrive and multiply and eventually create a turbidity in the lakes, which take on a green hue. Further, he said, the fish droppings are rich in ammonia, the most bioavailable of the nitrogen nutrients. So the fish transform nitrates and nitrites taken up by the algae into ammonia, ironically creating the best possible situation for algae growth. He concluded that, over the long haul, the fish defeat the purpose of controlling algae. Rafael will elaborate on this at subsequent PBSD meetings. Dave Trecker (12/3/14) MEETING WITH BETH JOHNSSEN AND DANETTE KINASZCZUK ON RECYCLED WATER (11/10/14) I met with Beth Johnssen, Director of Wastewater (Public Utilities Division), and Danette Kinaszczuk, Pollution Control Manager (Growth Management Division) to discuss recycled water in Pelican Bay. Two things, neither unexpected, came from the meeting. (1) Pelican Bay uses a large amount of recycled water for irrigation— 3.2- 3.5 million gallons per day for residents and businesses and 0.5 million for the golf course at The Club Pelican Bay. The target is 1.7- 1.9 million gallons per day for Pelican Bay based on the SFWMD guideline of 1 inch/acre-week. (2) The high levels of nutrients— 2.1 mg/L of dissolved nitrogen and 2.0 mg/L of dissolved phosphorus —in the recycled water were not challenged. The officials said the nutrients could be removed or reduced, but the cost would be prohibitive. I asked for historical data and breakdowns by areas in Pelican Bay, but I have received nothing yet. I will follow up. Dave Trecker (12/4/14) Tilapia Fact Sheet Genus,species: Oreochromis spp. (Gunther 1889), Sarotherodon spp.(Rupper 1852), Tilapia spp. (Smith 1840)(TSSG 2006)) Common Names: Tilapia(with a lower case"t"refers to all three species),boulti, freshwater snapper, mojara,ngege, St. Peter's fish (ISSG 2006). Taxonomic Synonyms: Oreochromis spp., Sarotherodon spp.. and Tilapia spp. inlcude roughly 70 species (ISSG 2006) . 4 Photo credit:MIT Sea Grant College Program. Tilapia are an economically important food fish that have the potential to out- compete native species in tropical environments across much of the southeastern United States. The species, blue tilapia, is the most abundant invasive fish species in the southeastern United States. They are successful aquaculture fish because they are hardy and easy to grow, white-fleshed, mild-flavored, and appeal to the palate of consumers. Life History: Similar to the grass carp,most tilapia species are herbivores that have the potential to alter aquatic plant populations and ecosystems. Tilapia are mouth brooders, which means eggs hatch in the mouth of the female,and the female protects the hatched young from predators in her mouth (GSMFC 2003). Means and Time of Introduction: From the 1980s,tilapia were introduced as aquaculture species that are often farmed in cages in open bodies of water. The fish can escape if the cage becomes damaged due to environmental forcing, such as hurricanes, storms,or human actions. Throughout the world,documented cases of tilapia introductions are frequently due to both release and escape (TSSG 2006). Blue tilapia (Oreochrmomis aureus)were introduced to Gulf states for weed control,in other cases it was for weed and insect control. They also have been released from aquariums and fish farms(GSMFC 2003). Origin: Tilapia is originally from the Middle East and Africa(ISSG 2006). North American Distribution: Blue Tilapia(Oreochromis aureus) can be found in Florida, Alabama, and Texas, although Alabama winters often do not allow survival of most populations (GSMFC 2003). Other tilapia species,many of which formed hybrids, are established in southern California irrigation ditches where they were introduced to control aquatic macrophytes. Habitat: Tilapia can be found in lakes,wetlands, marine habitats,water courses, estuaries, and marine environments. They prefer tropical environments with water temperatures in the 25-30°C range. Some species can tolerate cold temperatures down to the point of 8 or 9°C. Sensitivity to salinity also varies greatly between species; some species can fully tolerate seawater(ISSG 2006). Some species have been shown to tolerate salinities above 45 psu,but they may not reproduce at those salinities(GSMFC 2003). Ecological Impacts: Blue tilapia have become the most abundant invasive fish species in the Gulf states. Tilapia often compete with native species for the same type of food, and can therefore cause declines in native populations(GSMFC 2003). Tilapia that have escaped from aquaculture facilities may interbreed and form hybrids(Costa-Pierce 2003). Some species such as the Mozambique tilapia(Oreochromis mossambicus)have outcompeted native fish species and preyed on native larval fish in areas where it was introduced. Economic Impacts: Tilapia may compete with native fish for nesting space or food and thus have the potential to negatively impact native populations in warm environments (GSMFC 2003). Literature Cited: Costa-Pierce,Barry 2003. Rapid evolution of an established feral tilapia (Oreochromis spp.): the need to incorporate invasion science into regulatory structures. Biological Invasions 5: 71-84. GSMFC (Gulf States Marine Fisheries Commission) 2003. Fact Sheet for Oreochromis aureus. 21 November 2003. htto:.-nis.:`rAnt c.ori ni i'actsitwc:.pltp!tt i4_ 9. . Last accessed: 15 May 2006. ISSG(Invasive Species Specialist Group)2006. Ecology of Oreochromis spp. Global Invasive Species Database. 12 January 2006. ssg.orL datahasc specie: ecolu*.asp?si-813&fr=1&sts�s«. Last accessed: 15 May 2006. Additional References: FishBase 2006. Species Summaries for Tilapia. uam.fishbase.org. Last accessed: 15 May 2006. GSMFCb. Fact Sheet for Tilapia zilli. Gulf States Marine Fisheries Commission. 3 August 2005. http:%"nis.gsmfc.org%nis factsheet.php?toc_id=200. Last accessed: 15 May 2006. GSMFCc. Fact Sheet for Oreochromis mossambicus. Gulf States Marine Fisheries Commission. 3 August 2005. http:/Inis.gsmfc.org/nis factsheet.php?toc_td=195. Last accessed: 15 May 2006. GSMFCd. Fact Sheet for Tilapia mariae. Gulf States Marine Fisheries Commission. 3 August 2005. http:-!nis.gsmfc.org-n.is factsheet.php''toc id=199. Last accessed: 15 May 2006. Last Updated: 28 June 2006. Benefits Of Stocking Blue Tilapia • Blue Tilapia eat many of the common types of filamentous algae,blue green algae, chara, duckweed,watermeal, nuisance rooted aquatic vegetation.A perfect par with the amur who will not eat these types of algae • Prolific breeders • Fish spawn when they reach 4"\water temperature is above 68 F •Lay up to 1500 eggs per female • Spawn every 18 to 21 days •The babies are the ones that put the biggest strain on the algae\by converting vegetation into a bait fish for your predator fish. • Reduce demand on minnows and Amurs • Increase the size and population of other fish in your pond. • Fall temp cause fish to slow down allowing predator fish of all sizes to gorge itself just in time for winter • Control Muck • Reduce unpleasant gases • Most Eco Friendly solution to controlling algae, no more chemicals • Finally a solution from vegetation for pond owners who use their pond as a water supply. •A perfect substitution for the bluegills since they can not take over your pond. The list goes on but the time is now we only sell these the first part of June and we have secured a big supply however demand for the fish is great. Please call and place your order for these fish today. Fish supplied first come first serve bases Blue Tilapia Stocking Density Chart * Existing Size of Predator Fish.if no Large Mouth Amount of Bass was stocked than subtract 2"from the Pond predator size Covered by Stocked Lbs of - Aquatic- Tilapia per Acre * Less than *8"-10" *10"-12" *12"+ Vegetation of Water 8" Little 10-151bs Stock Stock Stock Stock Spotty 15-251bs All 75% 25% All 1 ft Around 25-501bs ¢ -6' 4"-6" 4"-6" 7"-!O' Pond 25% 75% Covered 50-100 lbs 7"-l0" 7"-10" W N N W Pi' n 3 r 0 a 0l M 03 fD W -P vi N X" 0 co ITi W O r N O o 0 N cn G) G) �► r+ r r" O ctn D o o ;� O • Uq Ora fD A-- fD 0 Z CD- CD CD vi B z u'►• CD o ° 3 fu 0� = = c cD a) v 0 O NJ NJ NJ NJ W ='+ NJ W C11 Cn V =� M: =` = ? = al CD CD fD CD CD H N9 to (J) to 0 c fD 3 fD 3 f . M. 03 A- N...) 1-a VI ti) u-1 00 UJ l!i a o 0 (11 (7,DJ -Ti r+ —I ° n au o 0 � et .Wi � v v � � -, -t+ 0 0 a -V). -cn. ih -cn N 0 001 . c NJ (..1.) O d -T, o n 1'" Q1 I-4 �I IV U, BEST SUPPRESSION OF ALGAE TO DATE Lake Treatment 3-5 Aeration + Plantings + Bacteria(1) 3-6 Aeration+Plantings + Bacteria (1) 3-7 Aeration+ Plantings +Bacteria(1) 3-1 Aeration+Plantings (2) 1-6 Aeration (2) 2-2 Plantings (2) 5-1 Tilapia (2) (1) Visual examinations in 2/14, 5/14, 9/14 and 11/14 (2) Visual examinations in 9/14 and 11/14 Observations • Combination of aeration +plantings + bacteria remains the best of the nine pilot treatments started 14-16 months ago. Bacteria may not be essential. • Tilapia treatment (5/14) appears to be effective • Some of the lakes with little or no algae have some duckweed along their perimeters. • There is no correlation between treatment effectiveness and nutrient levels, which remain high in many of the lakes which have little/no surface algae. COPPER LEVELS IN THE LAKES Copper (ug/L) Basin 3/13 8/13 11/13 2/14 5/14 8/14 1. 76 20 48 49 21 16 2 294 57 46 134 52 22 3 163 31 86 96 67 42 4 408 73 58 59 39 48 5 102 157 31 36 35 14 6 4.6 3.0 3.4 2.7 3.5 2.7 Between 11/13 and 8/14, measurable copper has ... • Declined in 34 lakes • Increased in 4 lakes • Remained the same in 5 lakes Copper remaining in lakes is due to ... • Incomplete run-off into Clam Bay ? • Equilibrium with copper in sediment ? AVERAGE COPPER LEVELS IN CLAM BAY, ug/L (1), (2) 2011 (6) 2012 (7) 2013 (8) 2014 (9) Outer Clam Bay (3) 1 .5 1 .6 4.0 3.3 Inner Clam Bay (4) 5.8 5.7 9.3 6.4 Upper Clam Bay (5) 9.2 8.9 7.3 6.0 (1) State limit = < 3.7 ug/L (2) Outliers (>40) excluded (3) Station CB6 for 2011/2012, stations W1 + W6 for 2013/2014 (4) Station CB3 for 2011/2012, station W7 for 2013/2014 (5) Station CB1 for 2011/2012, station UCB for 2013/2014 (6) Five monthly readings (7) Two monthly readings (8) Twelve monthly readings (9) Eight monthly readings to date Bad News: There has been no significant reduction in copper levels in Clam Bay since the FDEP measurements in 2011-12. Good News: There is a downward trend in Upper Clam Bay over 2011-2014, a slight downward trend in Inner Clam Bay in 2014, and consistently good readings (excluding an outlier) in Outer Clam Bay. This suggests tidal flushing may be starting to have an effect. Observations • Sampling stations were different in 2011/2012 and 2013/2014. • Inclusion or exclusion of outliers and the outlier limit chosen (>20, >40?) greatly affects average readings. How will FDEP handle outliers? _ _ _, , _ _ �' The Vacuum AirLift Multifunctional Water Treatment Tower Ca I e,, LICENSED PATENT INSA LYON I IFREMER J z �/ I ® Weiner Who We Are The Vacuum AirLift (VAL) technology is - ' " the six year culmination of research b and development by French research ' institutions, IFREMER and INSA. Multiple process patents protect the `, technology in the United States SEAREN - USA based LLC that holds the i. exclusive license of the technology in VAL demonstration the United States Pollutec-Paris December 2013 an .t.cT ,ap: ‘ 2 ,, s A Multi- Functional Technology Functions Energy Consumption WATER CIRCULATION 2-3 Wh/m3 of water Low pressure pump (3,000 to 3 million gpd) circulated GAS EXCHANGE 3- 10 Wh/m3 CO2, 02, N2, CH4, Radon, De-chlorination of water treated PARTICLE EXTRACTION .k 10-30 Wh/m3 0.3 to 90 microns of water treated SEAR Q _ The Technology s,__ frai 4i % ' a / `` -'''..'.'" '''''''..\ - 0 pi 4 ,,,,, , ,, _ 40,. , „,,.., ,.. . , t . ii ,,,...... . 4C,:,\.."-^^...'' '''''' '... ' .-- . 4,......--7t:t.r. =.., . ,r a.;vnyr A z i } .,,,„, , ,:v.N.4,4-, .,:e • , 4. 4 VAL Process -- ‹---• _: (1) Initial liquid 4 FOAM (2) VAL tower composed of it. 3��3 two concentric vertical tubes tft ((3) Vacuum pum which allows fi uid to rise in tie internal Foam tube (4) Harvest tank, to collect extracted elements. (micro bubbles inside the tower __J 4 17 L• WATERFLOW METER _ -I METER HARVE T TANK j __., C �)�i L 0 VACUUM �1 —'—�� �4�.._ - l3 f _. AIR lit SUPPLY 5 Particle Extraction 140 I . I 120 VI LL r ai U 100 8 /7.01".1.................. t `! o 80 O 0, N m 60 c , ; 0 40 , 20 0 0 10 20 30 40 • Efficiency increases with salinity Salinity Cho) Particle Extraction Range of separation size Microns 0.001 0.01 0.1 1.0 10 100 1000 Separation - Process * I f P" 'lfon Or!"Development Vacuum Airlift-1M Aqueous Salt Milk Proteins Clay Microalgae Relative Size of Sugars Viruses Bacteria Fat Micelles Common - I 1 Metal Ion Gelatin Blue Indigo Dye Cryptospondium Materials Synthetic Dyes Albumin Protein Asbestos Oil Emulsion Activated Carbon Microns 0.001 0.01 0.1 1.0 10 100 1000 SEAREN Qr.- 7 Bio—security "^ r Virus removal by foam fractionation (20nm) �� t Reduction of 3 logs 1 ELISA OD of virus versus time(mean of 4 Elisa tests) Virus particle number per ml sea water:mean of 4 PCR 0 25 results Q1.0E+07:. .__.__. .._...__. .__...... .. _. j0.20 •a I W 1.0E+00 -- • d 0 15 p E 1.0E+05-.. - •.. ... 0.0.10 O .�„ 1.0E+04-......_ _.... �. .. ... .. >0.05 • G 1.0E+03 0.00 .. _-.__...- - — -- 0 20 40 60 80 100 120 0 10 20 30 Time(min) Time(min) SEARS( 44 `- VAL Energy Consumption Energy/Suspended Solids Ratio 0 0 m c a, C v 0.1 ,3 Q vs ti n+ 0.01 NI VS 0.001 S Vacuum Gass Foam Chamber filter Tangential Nozzle Vacuum filters Decanter bowl Hydro cyclone Polymer Dissolved air otatiof.r flotation flow filtration discharge centrifuge flocculation flotation• ti f' centrifuge 5 Source: Thea Coward,Jonathan G.M.Lee,Gary S.Caldwell "Development of a foam flotation system for harvestine microaleae" In"Aleal Research"Vol.2.Is.2.March 2013.o135-144 VAL Specs Flow Rates Column Units Degassing Extraction Treatment ext. diam. built Mode Mode Capacity (mm) (gal./mn) (gal./mn) (gal./day) 25,000 to 300 12 100 17 150,000 100,000 to 600 25 400 70 600,000 500,000 to 1400 2 2,000 350 3,000,000 1,500,000 to 2 500 2015 In 5 6,000 1 ,000 8,500,000 10 Needs Depuration Cleans water(particle removal)and strips TSS reduction and degassing at dissolved gasses. VOC removal to be tested high water flow Oil &Gas 6. e. r : sac eria re.0 on, , s ripping Downstream: Oil &TSS reduction, gas stripping Sustainable solution Without additives for water treatment Aquaculture ter ., .6. .rw'. Efficient solution- CO2 stripping, particle removing, water circulating for CO2 stripping Microalgae •. imizi g e a gae pros uc ion process. To manage gases and to circulate water during the Reduce harvesting costs growing process and to harvest algae. to produce algae biomass a-s ----' 11 Purification (Oil & Gas, Waste Water, Desalinization) Af The VAL manages Upstream or Downstream water. ,, �„�:; ---- -- -__- GAS STRIPPING Gas management - ..... — - 02 CO2 N2 CH4.to 0 ppb i Ultra-filtration - . SDI REDUCTION ° 20 to< 3, in seawater r r � OIL EXTRACTION De-oiling t/ 500 to <20 ppm in seawater SEAREN wY-..,._ SDI & Dissolved Oxygen Results Silt Density Index (SDI) reduction: from 19 to 2.5 r 20 18 't 16 12 to I N2 % 4 \-^-.......„..,..„._...,._....,...___ ' 2 r Number of Cycles 0 1.67 333 5 73 10 15 20 40111.MI, Reduction of Oxygen: from 8,000 to 9 ppb . gig' -' Transmittance of the water: Final 97% of that of the ultra pure SDI: 2.5 s ,l#;-_- - OZ: 9 ppb .. it r . , ,,," �1 I�,a■ to l._,, �, .4 :-- .-..'t. -`— 13 Water De-Oiling Results 7 r N 1 ----q r¢ Extraction V 44- =@1; VAL' s Desalination Fit Ocean Water Si Er ' STEP 2 STEP 3 Pre-Treatment Reverse Osmosis Conditioning+diiislntiiectlnn i 4 4 f r( Pre-filtration: Post-treatment: micro-particles Degassing and de-oiling Higher efficiency for RO -Lower maintenance -Lower OPEX . iw i'EARE Application in purification rj;,- . i ,. . , , , I : ...., „,,,, vo •,. 4' . 9 \ I . 4 J;■ ----7- f . ., v j y 'i i vt ,.; ' .. cl fiti." It.y:,,, fp!, I ' ,i •. It ,-•• -i-, ,Iii,ik L.R1t ..:-.- . 7 ...,,ii. . 'k'\k, ii., - , i r ,tp.4vrtP ' ' . - i , 11 , 111,4 t'19`g 41 '• ,,ti aim - t,' : ,4a., �.4 , . . rlq II'`�°� �'�' 4 'r r 14,.I t+ .�� -- t ae�..w-,...., i-;,,�r 1 T 4 ,: AIM �f-,fir. / �i ^Sp .„ .. Landfill Leachate Trials 140 , is, , 4 , ' , 120 t."---o 100 4,200 mg/I t;42. go / ..."..'''''''''°*"...4 I\, r In 1/1000 i ...7. i.,, Low discharge, 1 of the initial i o 2! so c HS leachate's salinity ;t P CU c 0 40 :/ .... u , C., 0 20 This trial's salinity - j) Initial:250 mg/I : 0 10 20 30 40 . ., Final: 130 mg/I Salinity(%.) Or, _ '''-z,--;;--41'.:;:----3,;:t . _ . _ —– , -- Estimated Costs For 25 m3/h Treatment Capacity (1609000 gpd) . , Gas Particle . Function Total „St i_e ling . , ..Extraction. E 1 VAL 4 VALs VAL s 5 quipment (600 model) (1000 model) CAPEX Price* $30,000 $260,000 $290,000 Per year (10 years $3,000 $26,000 $29,000 deprec.) OPEX Energy** (6.32 cts/kwh) $9,412 $221 ,453 $230,865 (Per year) Maintenance $600 $6,000 $6,600 Total cost/year (with VAL) $13,012 $253,453 $266,465 Current cost/year(without VAL) ,= $2,920,000 tIrlil* *Pricing may vary based on specific setup requirements.**Energy may be reduced with optimization of the system. , .-... Wastewater + CO2 bioremediation pilot Water circulation and gas exchange system d I■ 1� `• �'` j ' T I � z� • i� , „, Tubular reactor z •� SEAREN _. 19 Micro-algae remediation performance Type of Examples Type of Treatment Comment pollutants treatment performance Nutrients CO2,NO3, NH4, Assimilation 80 a 100% Pilot testing PO4 Metals Toxic,rare, Biofixation 60 a 90% Lab results and radioactive publications on As,Cr,Cd,Cu,Mn, microalgae biofixation in Ni,Hg,Pb,U open systems HRAP Pharmaceuticals Diclofenac Photooxydative 100% Lab results and Sulfamethoxyazole degradation 60 a 100% publications Pesticides Thiamethoxam Photooxydative 100% Lab results and biodegradation publications Organic Bisphenol A Photooxydative 65% Lab results with selected micropollutants degradation microalgae strain Microorganisms Coliforms Photooxydative 4 log Pilot testing disinfection 99,99% . Low-density micro-algae harvesting in open ponds 22 • For a concentration of approx. 0.02 to 18 0.05 g/L DW is •Average harvest concentration of `s 12 - A approx. 2 g/L DW = 10 - E 8 . • Floc concentration comprised of 6 between 12 and 20 g/L DW ' 2 _: Culture Harvest Flocculation Concentration Factor (CF) after harvesting 20 to 100 CF after flocculation 300 to 800 Biomass in the floc 60%to 80%of total harvested biomass SEAREN 1 Harvest of Nannochloropsis Culture (4x) Harvest(4x) 1 CF> 170x Culture (40x) Harvest(40x) - • SEAREN Case study: Cost comparison 100 acre ponds Function: 1 Circulation/mixing CO2 dissolution 02 Harvesting +pumping stripping (14 H/day) A Paddle wheels Carbonating Equipment: +pumps stations ? DAF+thickeners CAPEX: $500,000 $250,000 ? $2,000,000 Energy: 1,000,000 kWh/Year 150,000 kW/Year ? 20,000,000 kWh/Year Equipment: 40 Vacuum Airlifts (diam. 2500 mm) Function: ✓ ✓ ✓ ✓ CAPEX: $2,800,000 Energy: 300,000 kWh/Year 1,500,000 kWh/Year OPEX reduction 90% energy reduction Pilot for HAB extraction (VAL 1400) * - .;: -_ i Water flow: 125 m 3/h - Initial algae concentration: 0. 1 - 0.2 g/I - Harvesting Flow: 50 I/h - Harvest concentration 10 g/I - Algae removal: 1 .1 Ib/h* = 26 Ib/d* f , ` +g iiEnergy consumption fix; 500 W/lb eq. DW* I *Equivalent dry weigh SEAREN ® ._-:.,,- The vacuum airlift as a production harvesting system • Process allowing to combine harvest, circulation and gas exchanges • Final product after pre-concentration sufficiently concentrated for final separation I Low density open pond Centrifugation drying + drying Vacuum airlift Efficient system for production and pre-concentration of open pond low-density microalgae with low energy consumption Advantages Multifunctional: gas stripping, foam fractionation, aeration and circulation Adaptable: on current tanks without modifications Scalable: capable of handling up to double production Security: comfortable margin of performance safety Evolutionary: VAL will evolve with the company's growth Upgradable: will easily accept product enhancement Additional advantages Control of Emissions: Extracted material (gases, liquids or solids) can be safely isolated or discharged. Low foot-print: Small ground space requirement No Additives: Purely physical process without consumables. Durable: Robustness and reliability (no moving parts) Anti-Clogging: Free passage, no clogging Resilient: Resilient system, can tolerate stoppages and restarts Simplicity: Simplicity of use and ease of maintenance Safety: Engine can be positioned away from the tanks. :w. =-'?, moz Searen Americas 4:0 Canada , `fx i � v. i --4 --,-77-74,- _.w! r WZ Cincinnati base h' Undid States '04,1,, ,n ..Q San Diego § , v - i 1 a? Naples i Business offices * e>� .- ..--;-- Al'.",) r.< . M �.w '' ri maneequipment Demonstration sites },; a.1.�. 6.)...+040 ....._ - •..• t Distributors N,arag,, awe < i. T W,. ?R VAL Operations and Projects J ........ I loMand Nt 3 RAS Sea 10 10 M5.5..5n MI-12 ' • 2q .5 COZ depung am 13 Canada 21 Feesh water RAS mane" Mar.14 0 ,,... 22 Mmt rerneat Ape 1.5 , / 23 CO2.TM renmed Aug.14 *- •-<, . „. 26 5415 CO2 depling /mg IM 27 MI tetanagmg #09 14 , UMed 25 K89d8 rn IIA3 CO2*pang 04.la PIVICO3 Fe re020.01 ' 2015 ,- '41'. tm no 4,,,.... CV Awe rems201 AO 05 s.s.r.-1....:,...:4 ..,-"\_,.._ „3"----...!• CO2 01 1,00. 2,17 *. --*-, ..... ,,,,,,`"....• ."3 Ism me lir 90 An ,.._ist" i/12.1116,, •tame it-m5"*,,,,,,,,,,,.,S;;:t . klitt .2. ., •• Untied Slates '''' ra '''' '''' ,,''. wee T.41. rl.:•,.1. %Omani I Samedaleddlasta--,,I 9 TSS renew! MI 12 - ...-5;;:i • /44_,''''',,,,,Z * .,.,_ .34 17 COO'atty.-non Sen-13 • .. ' °N ."‘ ,.. htYarets leadtanSte'treatment*5:210414 ' 147411;27;41 ,Ceoc:6.27- t'""\„, i"..":' 1*••''', Itnn ''' •194.5 I ltbya twat 5 Memo "• sato. t ',.. sand Arabia ' Dim Pueol4 Re* Oman eme.cntra _, , Malt 'Nig, , s.d. ,.„-....._ ,.. ,„.. ••••nm -,----+..- ''''A's.,r"""''-N ' '. Veretruels . ' i■,)Nlem, /•,:l. ,'.•,----;111(0.p.: 1111111111.111 D.. Colombo: ,---•9..... ' ', - Somalus Ramn, Dee.09 446cd<'-}.- - -3`: (.0,25 A Purikenon sdruhna mn 11 I RID I 7 %imam Feb 12 ,.. .' 2 Gas exchange•hydrols, An 10 r-2) DR ConDo 41 8 damming May 12 ( ,,,. aca 70,„,„ 1 Ultrahltraron Feb 11 13 Managing Mar 13 , .2 .., eon, , In . IIMI -14, Prele. CO2 removed *2015 16 Mararthng Apr 13 Pent '',. '' • . u 6 anct alma. Nem 11 15 Pelysamands cement An 15 II De tnemenatton Dec 12 M94.7 Zambia..Q. lir 19 CO2 rernedtaeon Mar le I GO 17 De 0 Ifing Dec 12 S ,.1. fr7.4.64* 29 CO2 IttilrellaeOn See le 24 Water Imatment Int 14 ' -h.b-'.' Pr NvnlIng 7212015 ' u uc ,. 970909 s ker10g 205 0 , * CO7 trmeekman ' 2023 ‘/-" pamitiiy, 50 04` * De OMMg 2015 805*088 --(' 0 29 - . Traction _ Scientific and tech support Si .„•• • ffSA Coldep , UNIVERSITY Of la Cincinnati Current users 24 VAL in operation Ifremer * microphgt A 1?D A HelwPur Les poissons Alb SA LI NS if;Nofirna -,C,. duSoleil. ibr ...., ,., ,......_ _ Integrators/ distributors agnmarine -+- marine equipment N...., Testing users ip,irt/if "......—. '....."'"...e.----z". ,- S a p p h i r e t KUTERRA V Energy SUSTAtx”ILITT IIIIS LARDED TOTAL Awards Cluster support ..c CleanTech ..-1:CT CONFLUENCE ____;,..............,-- 30 . . ..-.... • Due to its performance and simplicity, the VAL is a liquid-solid-gas exchange platform poised to become a standard in many applications. "Simplicity is the ultimate sophistication" Leonardo Da Vinci SEAREN 31 vhar • - u A 1 1r contact@ searen.com SEAREi .. 32 References: -Patents: • "Method and installation for treating an aqueous effluent, in order to extract at least one dissolved gaseous compound; application to aquaculture in recirculated aqueous medium." US Patent N°7 771515.June 20,2007. • "Micro particule extraction process » . US Patent file N° 075071, November 9, 2010. -Publications: • Barrut, B., Blancheton, J.P., Champagne, J.Y., Grasmick, A., 2012a. Mass transfer efficiency of a vacuum airlift - Application to water recycling in aquaculture - systems.Aquac.Eng.46, 18-26. • Barrut, B., Blancheton, J.P., Champagne, 1.Y., Grasmick, A. 2012b ater°del Stern capacity oft—Vacuum airlift Applic tiorr-to waterrecycling in aq IturVgl Aquac.Eng.48,31-39. x • Barrut;-B:, Blancheton, J.P., Muller-Feuga, A.,:Rene, F.; Narvaez, C:, Champagne, , 1.Y., Grasmick, A. Separation efficiency of a vacuum gaslift - Applicatior o microalgae harvesting.Bioresource Technology 128,235-240. • Barrut, B., Blancheton, J.P., Champagne, 1.Y., Grasmick, A. Foam fractionation efficiency of a vacuum airlift --Application to particulate matter removal in recirculating systems.Aquac. Eng.54, 16-21.